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IL-22 Antibody

ORB1239668
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIL22
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Overview

  • Supplier
    Biorbyt
  • Product Name
    IL-22 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    IL-22 antibody can be used for detection of IL-22 by Western blot at 0.5 microg/ml. Antibody can also be used for Immunocytochemistry starting at 5 microg/mL. For immunofluorescence start at 20 microg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.. IL-22 antibody can be used for detection of IL-22 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. ELISA, ICC, IF, WB
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID50616
  • Target name
    IL22
  • Target description
    interleukin 22
  • Target synonyms
    IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18, interleukin-22, IL-10-related T-cell-derived inducible factor, cytokine Zcyto18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZX6
  • Protein Name
    Interleukin-22
  • Scientific Description
    IL-22 Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL22 using anti-IL22 antibody (A00963-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 24 kDa. The expected band size for IL22 is at 20 kDa.
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