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ProductsBack/IL-32 Antibody (YA2911, Rabbit)

IL-32 Antibody (YA2911, Rabbit)

HY-P83166
MedChem Express
TargetIL32
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    IL-32 Antibody (YA2911, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9235
  • Target name
    IL32
  • Target description
    interleukin 32
  • Target synonyms
    IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, interleukin-32, interleukin-32 eta, interleukin-32 small, interleukin-32 theta, natural killer cell transcript 4, natural killer cells protein 4, tumor necrosis factor alpha-inducing factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    IL-32 Antibody (YA2911) is a non-conjugated IgG antibody, targeting IL-32, with a predicted molecular weight of 27 kDa (observed band size: 23 kDa). IL-32 Antibody (YA2911) can be used for WB experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL32 using anti-IL32 antibody (A03286). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL32 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL32 antigen affinity purified polyclonal antibody (Catalog # A03286) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL32 at approximately 19KD. The expected band size for IL32 is at 15KD.
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