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Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-IL-6 Polyclonal Antibody, Unconjugated (bs-0379R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-IL-6 Polyclonal Antibody, Unconjugated (bs-0379R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-IL-6 Polyclonal Antibody, Unconjugated (bs-0379R) followed by conjugation to the secondary antibody and DAB staining

IL-6 Polyclonal Antibody

BS-0379R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetIl6
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    IL-6 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID16193
  • Target name
    Il6
  • Target description
    interleukin 6
  • Target synonyms
    B-cell hybridoma growth factor; Il; Il-6; interleukin HP-1; interleukin-6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08505
  • Protein Name
    Interleukin-6
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • 20(S)-Ginsenoside Rg3-loaded electrospun membranes to prevent postoperative peritoneal adhesion. Qiu R et al., 2019 Aug 14, Biomed Microdevices
    Read more
  • Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis. Hou DD et al., 2019 Sep, Int Immunopharmacol
    Read more
  • Effect of splenectomy on attenuation of LPS-induced AKI through GTS-21-induced cholinergic anti-inflammatory pathway. Gao Y et al., 2019, Am J Transl Res
    Read more
  • Dexmedetomidine protects against lipopolysaccharide-induced sepsis-associated acute kidney injury via an alpha7 nAChR-dependent pathway. Kang K et al., 2018 Oct, Biomed Pharmacother
    Read more
  • Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice. Ding Y et al., 2018, Immun Ageing
    Read more
  • Expressions of IL-6, TNF-alpha and NF-kappaB in the skin of Chinese brown frog (Rana dybowskii). Xi L et al., 2017 Oct 19, Eur J Histochem
    Read more
  • Fluoxetine protects against methamphetamine-induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats. Wang Y et al., 2017 Feb, Mol Med Rep
    Read more
  • Djulis (Chenopodium formosanum Koidz.) Water Extract and Its Bioactive Components Ameliorate Dermal Damage in UVB-Irradiated Skin Models. Hong YH et al., 2016, Biomed Res Int
    Read more
  • C5aR inhibition in the early inflammatory phase does not affect bone regeneration in a model of uneventful fracture healing. Ehrnthaller C et al., 2016 Oct 26, Eur J Med Res
    Read more
  • Efficacy of azithromycin and metronidazole combined therapy on rats gingival overgrowth induced by cyclosporine-A: An experimental animal study. Elkabir MA et al., 2016 Sep-Dec, J Oral Biol Craniofac Res
    Read more

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Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (PB9034). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse J774A.1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates, Lane 3: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # PB9034) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
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