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ProductsBack/Purified Anti-Mouse IL-6 Antibody [MP5-20F3]

Purified Anti-Mouse IL-6 Antibody [MP5-20F3]

AGEL2383
Assay Genie
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIl6
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Purified Anti-Mouse IL-6 Antibody [MP5-20F3]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ICFCM, ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID16193
  • Target name
    Il6
  • Target description
    interleukin 6
  • Target synonyms
    B-cell hybridoma growth factor; Il; Il-6; interleukin HP-1; interleukin-6
  • Host
    Rat
  • Protein IDP08505
  • Protein Name
    Interleukin-6
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (PB9034). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse J774A.1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates, Lane 3: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # PB9034) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
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