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IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 beta stained by IL1 beta antibody (GTX130021) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).
IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 beta stained by IL1 beta antibody (GTX130021) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).
IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 beta stained by IL1 beta antibody (GTX130021) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).

IL1 beta antibody

GTX130021
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetIL1B
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL1 beta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.13 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3553
  • Target name
    IL1B
  • Target description
    interleukin 1 beta
  • Target synonyms
    IL-1, IL1-BETA, IL1F2, IL1beta, interleukin-1 beta, IL-1 beta, catabolin, interleukin 1beta, preinterleukin 1 beta, pro-interleukin-1-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01584
  • Protein Name
    Interleukin-1 beta
  • Scientific Description
    The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhang X, Lan Q, Zhang M, et al. Inhibition of AIM2 inflammasome activation by SOX/ORF37 promotes lytic replication of Kaposi's sarcoma-associated herpesvirus. Proc Natl Acad Sci U S A. 2023,120(27):e2300204120. doi: 10.1073/pnas.2300204120
    Read this paper
  • Jeong GU, Lee S, Kim DY, et al. Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model. J Virol. 2023,97(6):e0055623. doi: 10.1128/jvi.00556-23
    Read this paper
  • Lin CY, Huang KY, Kao SH, et al. Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response. Sci Adv. 2023,9(14):eade9944. doi: 10.1126/sciadv.ade9944
    Read this paper
  • Wei KC, Wei WJ, Liao CL, et al. Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1. Microbiol Spectr. 2023,11(1):e0358622. doi: 10.1128/spectrum.03586-22
    Read this paper
  • Law YY, Lee WF, Hsu CJ, et al. miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts. Aging (Albany NY). 2021,13(13):17227-17236. doi: 10.18632/aging.203201
    Read this paper
  • Tsai MH, Kuo CW, Lin TK, et al. Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population. Cells. 2020,9(8). doi: 10.3390/cells9081885
    Read this paper
  • Nam SM, Seo M, Seo JS, et al. Ascorbic Acid Mitigates D-galactose-Induced Brain Aging by Increasing Hippocampal Neurogenesis and Improving Memory Function. Nutrients. 2019,11(1). doi: 10.3390/nu11010176
    Read this paper

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Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate, Lane 2: rat spleen tissue lysate, Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
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