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IL1 beta antibody

GTX10750
GeneTex
ApplicationsWestern Blot, Neutralisation/Blocking
Product group Antibodies
TargetIl1b
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL1 beta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-2 microg/ml. Neutralizing/Inhibition: 50 pg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID16176
  • Target name
    Il1b
  • Target description
    interleukin 1 beta
  • Target synonyms
    IL-1beta, Il-1b, interleukin-1 beta, IL-1 beta, interleukin 1b
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP10749
  • Protein Name
    Interleukin-1 beta
  • Scientific Description
    The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hsu CG, Chávez CL, Zhang C, et al. The lipid peroxidation product 4-hydroxynonenal inhibits NLRP3 inflammasome activation and macrophage pyroptosis. Cell Death Differ. 2022,29(9):1790-1803. doi: 10.1038/s41418-022-00966-5
    Read this paper

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Figure 1. Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # A00101-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 36 kDa. The expected band size for IL1 beta is at 31 kDa.
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