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ProductsBack/IL10 antibody [JES5-2A5]

IL10 antibody [JES5-2A5]

GTX27379
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIl10
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL10 antibody [JES5-2A5]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    JES5-2A5
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16153
  • Target name
    Il10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF, If2a, Il-10, interleukin-10, cytokine synthesis inhibitory factor, interferon 2a
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP18893
  • Protein Name
    Interleukin-10
  • Scientific Description
    This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates its immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • The Generation of an Engineered Interleukin-10 Protein With Improved Stability and Biological Function. Minshawi F et al., 2020, Front Immunol
    Read more
  • The p38 mitogen-activated protein kinases can have opposing roles in the antigen-dependent or endotoxin-stimulated production of IL-12 and IFN-gamma. Salmon RA et al., 2001 Nov, Eur J Immunol
    Read more

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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