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Western Blot analysis of Hela cells using IL-12Rbeta1 Polyclonal Antibody
Western Blot analysis of Hela cells using IL-12Rbeta1 Polyclonal Antibody
Western Blot analysis of Hela cells using IL-12Rbeta1 Polyclonal Antibody

IL12RB1 Antibody

CSB-PA006274
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIL12RB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL12RB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3594
  • Target name
    IL12RB1
  • Target description
    interleukin 12 receptor subunit beta 1
  • Target synonyms
    CD212, IL-12R-BETA1, IL12RB, IMD30, interleukin-12 receptor subunit beta-1, IL-12 receptor beta component, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, cluster of differentiation 212, interleukin 12 receptor, beta 1, interleukin-12 receptor beta-1 chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42701
  • Protein Name
    Interleukin-12 receptor subunit beta-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL12RB1 using anti-IL12RB1 antibody (A03401-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12RB1 antigen affinity purified polyclonal antibody (Catalog # A03401-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12RB1 at approximately 70KD. The expected band size for IL12RB1 is at 73KD.
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