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Anti-SHH Antibody

CAB18863
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIL12RB1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-SHH Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID3594
  • Target name
    IL12RB1
  • Target description
    interleukin 12 receptor subunit beta 1
  • Target synonyms
    CD212, IL-12R-BETA1, IL12RB, IMD30, interleukin-12 receptor subunit beta-1, IL-12 receptor beta component, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, cluster of differentiation 212, interleukin 12 receptor, beta 1, interleukin-12 receptor beta-1 chain
  • Host
    Rabbit
  • Protein IDP42701
  • Protein Name
    Interleukin-12 receptor subunit beta-1
  • Scientific Description
    SHH Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of IL12RB1 using anti-IL12RB1 antibody (A03401-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12RB1 antigen affinity purified polyclonal antibody (Catalog # A03401-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12RB1 at approximately 70KD. The expected band size for IL12RB1 is at 73KD.
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