IL13 Antibody (Azide-free)

LifeSpan BioSciences

IL13 Antibody (Azide-free)

23

ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.. ELISA, Flo, IF, Neut, SwELISA, WB ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

Unconjugated

...

IL13

interleukin 13

IL-13, P600, interleukin-13

Rat

IgG1

-20°C,2°C to 8°C

12352203