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ProductsBack/IL17B Antibody (aa77-89)

IL17B Antibody (aa77-89)

LS-C186527
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIL17B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL17B Antibody (aa77-89)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 20kD band observed in Human Ileum lysates (calculated MW of 20.4kD according to NP_055258.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 17kD. This band was successfully blocked by incubation with the immunizing peptide.. Peptide-ELISA (1:128000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 20kD band observed in Human Ileum lysates (calculated MW of 20.4kD according to NP_055258.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 17kD. This band was successfully blocked by incubation with the immunizing peptide.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27190
  • Target name
    IL17B
  • Target description
    interleukin 17B
  • Target synonyms
    IL-17B, IL-20, NIRF, ZCYTO7, interleukin-17B, cytokine-like protein ZCYTO7, interleukin 20, interleukin-17 beta, neuronal interleukin-17 related factor
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
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