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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

IL17C Antibody

CSB-PA301181
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL17C
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL17C Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27189
  • Target name
    IL17C
  • Target description
    interleukin 17C
  • Target synonyms
    CX2, IL-17C, interleukin-17C, cytokine CX2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0M4
  • Protein Name
    Interleukin-17C
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL17C using anti-IL17C antibody (A00164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17C antigen affinity purified polyclonal antibody (Catalog # A00164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17C at approximately 29KD. The expected band size for IL17C is at 22KD.
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