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Western blot analysis of extracts from LOVO cells, using IL17RAantibody.
Western blot analysis of extracts from LOVO cells, using IL17RAantibody.
Western blot analysis of extracts from LOVO cells, using IL17RAantibody.

IL17RA Antibody

CSB-PA058027
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIL17RA
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL17RA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23765
  • Target name
    IL17RA
  • Target description
    interleukin 17 receptor A
  • Target synonyms
    CANDF5, CD217, CDw217, IL-17RA, IL17R, IMD51, hIL-17R, interleukin-17 receptor A, IL-17 receptor A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96F46
  • Protein Name
    Interleukin-17 receptor A
  • Scientific Description
    Receptor for IL17A, IL17F and, in dimer with IL17RE, for IL17C. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling. Dunham I., Genome Biol. 5:R84.1-R84.11(2004). Venter J.C., Submitted (JUL-2005). Henzel W.J., Protein Sci. 13:2819-2824(2004).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Raw264.7 cell lysates probed with IL17RA/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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References
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WB analysis of A-20 cell lysate using GTX16598 IL17 Receptor alpha antibody. Working concentration : 1 microg/ml
Product group Antibodies
References
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ReactivityHuman, Mouse, Rat
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Western blot analysis in human cell line THP-1.
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Figure 1. Western blot analysis of IL17RA using anti-IL17RA antibody (A01311-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate,Lane 3: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17RA antigen affinity purified polyclonal antibody (Catalog # A01311-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17RA at approximately 160KD. The expected band size for IL17RA is at 96KD.
Product group Antibodies
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TargetIL17RA
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ReactivityHuman
TargetIL17RA
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