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Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: RAW264.7 cells, Primary antibody: CSB-PA871420(IL18RAP Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: RAW264.7 cells, Primary antibody: CSB-PA871420(IL18RAP Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: RAW264.7 cells, Primary antibody: CSB-PA871420(IL18RAP Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute

IL18RAP Antibody

CSB-PA871420
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetIL18RAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL18RAP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8807
  • Target name
    IL18RAP
  • Target description
    interleukin 18 receptor accessory protein
  • Target synonyms
    ACPL, CD218b, CDw218b, IL-18R-beta, IL-18RAcP, IL-18Rbeta, IL-1R-7, IL-1R7, IL-1RAcPL, IL18RB, interleukin-18 receptor accessory protein, CD218 antigen-like family member B, IL-18 receptor accessory protein, IL-18 receptor beta, cluster of differentiation w218b, interleukin-1 receptor 7, interleukin-18 receptor beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95256
  • Protein Name
    Interleukin-18 receptor accessory protein
  • Scientific Description
    Interleukin 18 receptor accessory protein, also known as IL18RAP and CDw218b (cluster of differentiationw218b), is a human gene. The protein encoded by this gene is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL-18R Beta/IL-18RAP using anti-IL-18R Beta/IL-18RAP antibody (A06457-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-18R Beta/IL-18RAP antigen affinity purified polyclonal antibody (Catalog # A06457-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-18R Beta/IL-18RAP at approximately 72KD. The expected band size for IL-18R Beta/IL-18RAP is at 72KD.
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