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Il1b Antibody Pair

CSB-EAP17347
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Goat, Human, Mouse, Porcine
TargetIl1b
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Overview

  • Supplier
    Cusabio
  • Product Name
    Il1b Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.2ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID16176
  • Target name
    Il1b
  • Target description
    interleukin 1 beta
  • Target synonyms
    IL-; IL-1 beta; Il-1b; IL-1beta; interleukin-1 beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10749
  • Protein Name
    Interleukin-1 beta
  • Reactivity
    Bovine, Goat, Human, Mouse, Porcine
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # A00101-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 36 kDa. The expected band size for IL1 beta is at 31 kDa.
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