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IL1F10 Antibody

LS-C748196
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetIL1F10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL1F10 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 16kDa, while the observed MW by Western blot was 17kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 16kDa, while the observed MW by Western blot was 17kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84639
  • Target name
    IL1F10
  • Target description
    interleukin 1 family member 10
  • Target synonyms
    FIL1-theta, FKSG75, IL-1HY2, IL-38, IL1-theta, IL1HY2, interleukin-1 family member 10, FIL1 theta, IL-1 theta, IL-1F10 (canonical form IL-1F10a), family of interleukin 1-theta, interleukin 1 family member 10 (theta), interleukin-1 HY2, interleukin-1 receptor antagonist FKSG75, interleukin-1 receptor antagonist-like FIL1 theta, interleukin-38
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL1F10 using anti-IL1F10 antibody (A09268-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87MG whole cell lysates, Lane 2: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1F10 antigen affinity purified polyclonal antibody (Catalog # A09268-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1F10 at approximately 17KD. The expected band size for IL1F10 is at 17KD.
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