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IL1F10 Monoclonal Antibody

CAC12981
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIL1F10
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Overview

  • Supplier
    Biomatik
  • Product Name
    IL1F10 Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A2B3
  • Conjugate
    Unconjugated
  • Gene ID84639
  • Target name
    IL1F10
  • Target description
    interleukin 1 family member 10
  • Target synonyms
    family of interleukin 1-theta; FIL1 theta; FIL1-theta; FKSG75; IL-1 theta; IL-1F10 (canonical form IL-1F10a); IL1HY2; IL-1HY2; IL1-theta; IL-38; interleukin 1 family member 10 (theta); interleukin-1 family member 10; interleukin-1 HY2; interleukin-1 receptor antagonist FKSG75; interleukin-1 receptor antagonist-like FIL1 theta; interleukin-38
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8WWZ1
  • Protein Name
    Interleukin-1 family member 10
  • Scientific Description
    The IL1F10 Monoclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL1F10 using anti-IL1F10 antibody (A09268-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87MG whole cell lysates, Lane 2: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1F10 antigen affinity purified polyclonal antibody (Catalog # A09268-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1F10 at approximately 17KD. The expected band size for IL1F10 is at 17KD.
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