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Western Blot analysis of Human fetal intestine tissue using ST2 Polyclonal Antibody at dilution of 1:700
Western Blot analysis of Human fetal intestine tissue using ST2 Polyclonal Antibody at dilution of 1:700
Western Blot analysis of Human fetal intestine tissue using ST2 Polyclonal Antibody at dilution of 1:700

ST2 Polyclonal Antibody

E-AB-10683
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetIL1RL1
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Overview

  • Supplier
    Elabscience
  • Product Name
    ST2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9173
  • Target name
    IL1RL1
  • Target description
    interleukin 1 receptor like 1
  • Target synonyms
    DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1, interleukin-1 receptor-like 1, growth stimulation-expressed, homolog of mouse growth stimulation-expressed, interleukin 1 receptor-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01638
  • Protein Name
    Interleukin-1 receptor-like 1
  • Scientific Description
    The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. Alternative splicing of this gene results in multiple transcript variants.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ST2/IL1RL1 using anti-ST2/IL1RL1 antibody (A00492-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST2/IL1RL1 antigen affinity purified polyclonal antibody (Catalog # A00492-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST2/IL1RL1 at approximately 70 kDa. The expected band size for ST2/IL1RL1 is at 63 kDa.
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