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ProductsBack/IL2 Receptor alpha antibody [PC61.5] (PE-Cy7)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-10 IL2 Receptor alpha antibody [PC61.5] (PE-Cy7). Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (PE-Cy7) and Mouse CD4 antibody (APC) Left panel : co-stained with isotype control and Mouse CD4 antibody (APC) antibody amount : 0.125 μg (5 μl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-10 IL2 Receptor alpha antibody [PC61.5] (PE-Cy7). Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (PE-Cy7) and Mouse CD4 antibody (APC) Left panel : co-stained with isotype control and Mouse CD4 antibody (APC) antibody amount : 0.125 μg (5 μl)
FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-10 IL2 Receptor alpha antibody [PC61.5] (PE-Cy7). Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (PE-Cy7) and Mouse CD4 antibody (APC) Left panel : co-stained with isotype control and Mouse CD4 antibody (APC) antibody amount : 0.125 μg (5 μl)

IL2 Receptor alpha antibody [PC61.5] (PE-Cy7)

GTX01472-10
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetIl2ra
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL2 Receptor alpha antibody [PC61.5] (PE-Cy7)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PC61.5
  • Concentration
    0.2 mg/ml
  • Conjugate
    Double Conjugated
  • Gene ID16184
  • Target name
    Il2ra
  • Target description
    interleukin 2 receptor, alpha chain
  • Target synonyms
    CD25, Il2r, Ly-43, interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R alpha chain, IL-2R subunit alpha, IL2-RA, p55 chain
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDP01590
  • Protein Name
    Interleukin-2 receptor subunit alpha
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 Receptor alpha/Il2ra antigen affinity purified polyclonal antibody (Catalog # A00214-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 Receptor alpha/Il2ra at approximately 50 kDa. The expected band size for IL-2 Receptor alpha/Il2ra is at 50 kDa.
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Lane 1: EC9706 lysates; Lane 2: mouse lymph node lysates probed with IL2RA Polyclonal Antibody, Unconjugated (bs-0577R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
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FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-02 IL2 Receptor alpha antibody [PC61.5] (Biotin). Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (Biotin) and Mouse CD4 antibody (APC) Left panel : co-stained with isotype control and Mouse CD4 antibody (APC) antibody amount : 0.125 μg (5 μl)
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FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-06 IL2 Receptor alpha antibody [PC61.5] (FITC). Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (FITC) and Mouse CD4 antibody (APC) Left panel : co-stained with isotype control and Mouse CD4 antibody (APC) antibody amount : 0.125 μg (5 μl)
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FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-08 IL2 Receptor alpha antibody [PC61.5] (PE). Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (PE) and Mouse CD4 antibody (APC) Left panel : co-stained with isotype control and Mouse CD4 antibody (APC) antibody amount : 0.125 μg (5 μl)
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