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IL22 Antibody (YA4481, Mouse)

HY-P84784
MedChem Express
TargetIL22
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    IL22 Antibody (YA4481, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID50616
  • Target name
    IL22
  • Target description
    interleukin 22
  • Target synonyms
    IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18, interleukin-22, IL-10-related T-cell-derived inducible factor, cytokine Zcyto18
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    IL22 Antibody (YA4481) is a mouse-derived and non-conjugated IgG2a antibody, targeting to IL22. It can be applicated for IHC-P, ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL22 using anti-IL22 antibody (A00963-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 24 kDa. The expected band size for IL22 is at 20 kDa.
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