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ProductsBack/IL22 ELISA pair [MT230/MT231]
Sandwich ELISA analysis of mouse IL-22 protein using GTX02980 IL22 antibody [MT230] as coating antibody and GTX02981-02 IL22 antibody [MT231] (Biotin) as detecting antibody.
Sandwich ELISA analysis of mouse IL-22 protein using GTX02980 IL22 antibody [MT230] as coating antibody and GTX02981-02 IL22 antibody [MT231] (Biotin) as detecting antibody.
Sandwich ELISA analysis of mouse IL-22 protein using GTX02980 IL22 antibody [MT230] as coating antibody and GTX02981-02 IL22 antibody [MT231] (Biotin) as detecting antibody.

IL22 ELISA pair [MT230/MT231]

GTX03085
GeneTex
ApplicationsELISA
Product group Antibodies
ReactivityMouse
TargetIL22
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL22 ELISA pair [MT230/MT231]
  • Delivery Days Customer
    9
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Gene ID50616
  • Target name
    IL22
  • Target description
    interleukin 22
  • Target synonyms
    IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18, interleukin-22, IL-10-related T-cell-derived inducible factor, cytokine Zcyto18
  • Protein IDQ9GZX6
  • Protein Name
    Interleukin-22
  • Scientific Description
    This gene is a member of the IL10 family of cytokines that mediate cellular inflammatory responses. The encoded protein functions in antimicrobial defense at mucosal surfaces and in tissue repair. This protein also has pro-inflammatory properties and plays a role in in the pathogenesis of several intestinal diseases. [provided by RefSeq, Jul 2018]
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116163

Datasheet

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Figure 1. Western blot analysis of IL22 using anti-IL22 antibody (A00963-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 24 kDa. The expected band size for IL22 is at 20 kDa.
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