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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA875653ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA875653ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA875653ESR1HU at dilution of 1:100

IL25 Antibody

CSB-PA875653ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL25
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL25 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64806
  • Target name
    IL25
  • Target description
    interleukin 25
  • Target synonyms
    IL17E; interleukin-17E; interleukin-25
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H293
  • Protein Name
    Interleukin-25
  • Scientific Description
    Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL17E using anti-IL17E antibody (A04429-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL25 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17E antigen affinity purified polyclonal antibody (Catalog # A04429-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17E at approximately 17KD. The expected band size for IL17E is at 17KD.
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