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WB analysis of mouse testis tissue using GTX53468 IL17E antibody [68C1039.2]. Dilution : 2 microg/ml
WB analysis of mouse testis tissue using GTX53468 IL17E antibody [68C1039.2]. Dilution : 2 microg/ml
WB analysis of mouse testis tissue using GTX53468 IL17E antibody [68C1039.2]. Dilution : 2 microg/ml

IL17E antibody [68C1039.2]

GTX53468
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL25
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL17E antibody [68C1039.2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100 - 1:250. IHC-P: 1:100 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    68C1039.2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64806
  • Target name
    IL25
  • Target description
    interleukin 25
  • Target synonyms
    IL17E; interleukin-17E; interleukin-25
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H293
  • Protein Name
    Interleukin-25
  • Scientific Description
    The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. This cytokine can induce NF-kappaB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17BR. Studies of the similar gene in mice suggested that this cytokine may be a proinflammatory cytokine favoring Th2-type immune response. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17E using anti-IL17E antibody (A04429-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL25 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17E antigen affinity purified polyclonal antibody (Catalog # A04429-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17E at approximately 17KD. The expected band size for IL17E is at 17KD.
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