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ProductsBack/IL27 antibody [11B29]

IL27 antibody [11B29]

GTX53349
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetIl27
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL27 antibody [11B29]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11B29
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID246779
  • Target name
    Il27
  • Target description
    interleukin 27
  • Target synonyms
    IL-27, IL-27-A, IL-27p28, IL27-A, Il30, p28, interleukin-27 subunit alpha, IL-27 p28 subunit, IL-27 subunit alpha, interleukin 30
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDQ8K3I6
  • Protein Name
    Interleukin-27 subunit alpha
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of IL27 using anti-IL27 antibody (A00857-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL27 antigen affinity purified polyclonal antibody (Catalog # A00857-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL27 at approximately 29KD. The expected band size for IL27 is at 27KD.
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