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IHC-P analysis of human spleen tissue using GTX85070 IL32 antibody. Working concentration : 10 microg/ml
IHC-P analysis of human spleen tissue using GTX85070 IL32 antibody. Working concentration : 10 microg/ml
IHC-P analysis of human spleen tissue using GTX85070 IL32 antibody. Working concentration : 10 microg/ml

IL32 antibody

GTX85070
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL32
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Overview

  • Supplier
    GeneTex
  • Product Name
    IL32 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 5 - 10 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9235
  • Target name
    IL32
  • Target description
    interleukin 32
  • Target synonyms
    IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cell transcript 4; natural killer cells protein 4; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; tumor necrosis factor alpha-inducing factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24001
  • Protein Name
    Interleukin-32
  • Scientific Description
    Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2 . It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32 (2). IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-alpha, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNgamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis (3).
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL32 using anti-IL32 antibody (A03286). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL32 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL32 antigen affinity purified polyclonal antibody (Catalog # A03286) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL32 at approximately 19KD. The expected band size for IL32 is at 15KD.
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