Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody (GTX48714). Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4oC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody (GTX48714). Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4oC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
IL7 Receptor alpha (phospho Tyr449) antibody
GTX48714
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIl7r
Overview
- SupplierGeneTex
- Product NameIL7 Receptor alpha (phospho Tyr449) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:10000. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.72 mg/ml
- ConjugateUnconjugated
- Gene ID16197
- Target nameIl7r
- Target descriptioninterleukin 7 receptor
- Target synonymsCD127; IL-; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7RA; IL-7Ralpha; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin-7 receptor subunit alpha
- HostRabbit
- IsotypeIgG
- Protein IDP16872
- Protein NameInterleukin-7 receptor subunit alpha
- Scientific DescriptionInterleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203