iLite® GM-CSF*

Svar Life Science

iLite® GM-CSF*

9

Assay Ready Cells

Research Use Only

The iLite® GM-CSF Assay Ready Cells can be used for the quantification GM-CSF activity, GM-CSF inhibitor activity, and for determination of neutralizing antibodies against such either in buffer systems or human serum. As a functional assay, iLite GM-CSF assay ready cells can be used to determine the activity of GM-CSF, or of GM-CSF inhibitors, as well as neutralizing antibodies, all using the same assay set-up. The assay ready iLite GM-CSF cells are a genetically engineered reporter gene cell line (U937) responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

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