Immunofluorescence staining of MCF-7 cells with CSB-PA00999A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ILK Antibody
CSB-PA00999A0RB
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetILK
Overview
- SupplierCusabio
- Product NameILK Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID3611
- Target nameILK
- Target descriptionintegrin linked kinase
- Target synonyms59 kDa serine/threonine-protein kinase; beta-integrin-linked kinase; epididymis secretory protein Li 28; HEL-S-28; ILK-1; ILK-2; integrin-linked kinase-2; integrin-linked protein kinase; P59; p59ILK
- HostRabbit
- IsotypeIgG
- Protein IDQ13418
- Protein NameIntegrin-linked protein kinase
- Scientific DescriptionReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203