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ProductsBack/ILK antibody [N1C1]

ILK antibody [N1C1]

GTX101691
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetILK
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Overview

  • Supplier
    GeneTex
  • Product Name
    ILK antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3611
  • Target name
    ILK
  • Target description
    integrin linked kinase
  • Target synonyms
    59 kDa serine/threonine-protein kinase; beta-integrin-linked kinase; epididymis secretory protein Li 28; HEL-S-28; ILK-1; ILK-2; integrin-linked kinase-2; integrin-linked protein kinase; P59; p59ILK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13418
  • Protein Name
    Integrin-linked protein kinase
  • Scientific Description
    Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Upregulation of integrin-linked kinase enhances tumor progression in gemcitabine-resistant pancreatic cancer.
    Read more
  • Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells. Chen YL et al., 2021 Jan 12, Sci Rep
    Read more
  • The Impact of Integrin-Mediated Matrix Adhesion on Cisplatin Resistance of W1 Ovarian Cancer Cells. Wantoch von Rekowski K et al., 2019 Nov 26, Biomolecules
    Read more
  • Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin beta1. Yen YC et al., 2015 Dec 8, Oncotarget
    Read more
  • Novel link of anti-apoptotic ATF3 with pro-apoptotic CTMP in the ischemic brain. Huang CY et al., 2015 Apr, Mol Neurobiol
    Read more

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Figure 1. Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (A02932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whhole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U20S whhole cell lysates, Lane 6: human PC-3 whhole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat PC-12 whhole cell lysates, Lane 11: mouse heart tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse skeletal muscle tissue lysates, Lane 14: mouse C2C12 whhole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin linked ILK antigen affinity purified polyclonal antibody (Catalog # A02932-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 51 kDa. The expected band size for Integrin linked ILK is at 51 kDa.
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