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ProductsBack/ILK antibody [N1C1]
Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)
Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

ILK antibody [N1C1]

GTX101691
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetILK
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Overview

  • Supplier
    GeneTex
  • Product Name
    ILK antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3611
  • Target name
    ILK
  • Target description
    integrin linked kinase
  • Target synonyms
    HEL-S-28, ILK-1, ILK-2, P59, p59ILK, scaffold protein ILK, 59 kDa serine/threonine-protein kinase, beta-integrin-linked kinase, epididymis secretory protein Li 28, inactive integrin-linked kinase, integrin-linked kinase-2, integrin-linked protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13418
  • Protein Name
    Scaffold protein ILK
  • Scientific Description
    Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Murase H, Matsuo Y, Denda Y, et al. Upregulation of integrin‑linked kinase enhances tumor progression in gemcitabine‑resistant pancreatic cancer. Oncol Rep. 2023,50(3):pii: 164. doi: 10.3892/or.2023.8601.
    Read this paper
  • Chen YL, Yen YC, Jang CW, et al. Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells. Sci Rep. 2021,11(1):644. doi: 10.1038/s41598-020-80060-3
    Read this paper
  • Wantoch von Rekowski K, König P, Henze S, et al. The Impact of Integrin-Mediated Matrix Adhesion on Cisplatin Resistance of W1 Ovarian Cancer Cells. Biomolecules. 2019,9(12). doi: 10.3390/biom9120788
    Read this paper
  • Yen YC, Hsiao JR, Jiang SS, et al. Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin β1. Oncotarget. 2015,6(39):41837-55. doi: 10.18632/oncotarget.5995
    Read this paper
  • Huang CY, Chen JJ, Wu JS, et al. Novel link of anti-apoptotic ATF3 with pro-apoptotic CTMP in the ischemic brain. Mol Neurobiol. 2015,51(2):543-57. doi: 10.1007/s12035-014-8710-0
    Read this paper

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Figure 1. Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (A02932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whhole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U20S whhole cell lysates, Lane 6: human PC-3 whhole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat PC-12 whhole cell lysates, Lane 11: mouse heart tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse skeletal muscle tissue lysates, Lane 14: mouse C2C12 whhole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin linked ILK antigen affinity purified polyclonal antibody (Catalog # A02932-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 51 kDa. The expected band size for Integrin linked ILK is at 51 kDa.
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