Bio-Connect

IMPDH1 antibody [N1C1]

GTX102814
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetIMPDH1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    IMPDH1 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3614
  • Target name
    IMPDH1
  • Target description
    inosine monophosphate dehydrogenase 1
  • Target synonyms
    IMP (inosine 5'-monophosphate) dehydrogenase 1; IMP (inosine monophosphate) dehydrogenase 1; IMPD; IMPD 1; IMPD1; IMPDH 1; IMPDH-I; inosine-5'-monophosphate dehydrogenase 1; LCA11; RP10; sWSS2608
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20839
  • Protein Name
    Inosine-5'-monophosphate dehydrogenase 1
  • Scientific Description
    The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
TargetIMPDH1
  • SizePrice
IHC image of CSB-PA22459A0Rb diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
IMPDH1 AntibodyCSB-PA22459A0RB
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetIMPDH1
  • SizePrice
WB analysis of various sample lysates using GTX66572 IMPDH1 antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetIMPDH1
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetIMPDH1
  • SizePrice
Anti-IMPDH1 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • SizePrice
Figure 1. Western blot analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMPDH1 antigen affinity purified polyclonal antibody (Catalog # A03791-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMPDH1 at approximately 55 kDa. The expected band size for IMPDH1 is at 55 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIMPDH1
  • SizePrice
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IMPDH1 Polyclonal Antibody(bs-6256R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetIMPDH1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IMPDH1 AntibodyLS-C403248
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetIMPDH1
  • SizePrice