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ProductsBack/Indoleamine 2,3-dioxygenase Antibody (YA3100, Rabbit)

Indoleamine 2,3-dioxygenase Antibody (YA3100, Rabbit)

HY-P83355
MedChem Express
TargetIDO1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Indoleamine 2,3-dioxygenase Antibody (YA3100, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3620
  • Target name
    IDO1
  • Target description
    indoleamine 2,3-dioxygenase 1
  • Target synonyms
    IDO, IDO-1, INDO, indoleamine 2,3-dioxygenase 1, indolamine 2,3 dioxygenase, indole 2,3-dioxygenase, indoleamine-pyrrole 2,3-dioxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Indoleamine 2,3-dioxygenase Antibody (YA3100) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3100), targeting Indoleamine 2,3-dioxygenase, with a predicted molecular weight of 45 kDa (observed band size: 45 kDa). Indoleamine 2,3-dioxygenase Antibody (YA3100) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IDO1 using anti-IDO1 antibody (PB9603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO1 antigen affinity purified polyclonal antibody (Catalog # PB9603) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO1 at approximately 45KD. The expected band size for IDO1 is at 45KD.
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