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ING1 Rabbit pAb

A7904
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetING1
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Overview

  • Supplier
    ABclonal
  • Product Name
    ING1 Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3621
  • Target name
    ING1
  • Target description
    inhibitor of growth family member 1
  • Target synonyms
    p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a, inhibitor of growth protein 1, growth inhibitor ING1, growth inhibitory protein ING1, tumor suppressor ING1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UK53
  • Protein Name
    Inhibitor of growth protein 1
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ING1 using anti-ING1 antibody (PB9644). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING1 antigen affinity purified polyclonal antibody (Catalog # PB9644) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING1 at approximately 47 kDa. The expected band size for ING1 is at 47 kDa.
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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
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