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ING2 Polyclonal Antibody

BS-16653R
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetING2
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Overview

  • Supplier
    Bioss
  • Product Name
    ING2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3622
  • Target name
    ING2
  • Target description
    inhibitor of growth family member 2
  • Target synonyms
    ING1L, p33ING2, inhibitor of growth protein 2, ING1Lp, inhibitor of growth 1-like protein, p32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H160
  • Protein Name
    Inhibitor of growth protein 2
  • Reactivity
    Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ING2 using anti-ING2 antibody (A04290-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse EL-4 whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING2 antigen affinity purified polyclonal antibody (Catalog # A04290-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING2 at approximately 37 kDa. The expected band size for ING2 is at 33 kDa.
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