![Western blot using GeneTexs purified anti-p47 ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 microg of RKO whole cell lysate separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4o C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L] MXHur for 45 min at room temperature. IRDye?800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48488/GTX48488_20160330_WB_w_23060823_856.webp "Western blot using GeneTexs purified anti-p47 ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 microg of RKO whole cell lysate separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4o C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L] MXHur for 45 min at room temperature. IRDye?800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
ING3 antibody
GTX48488
Overview
- SupplierGeneTex
- Product NameING3 Antibody
- Delivery Days Customer9
- Antibody SpecificityReactivity occurs against human p47 ING3 protein. This sequence only shows homology to isoform 1 for ING3. The sequence is not present in isoforms 2 and 3.
- Application Supplier NoteWB: 1:200-1:2000. ELISA: 1:10000-1:40000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.1 mg/ml
- ConjugateUnconjugated
- Gene ID54556
- Target nameING3
- Target descriptioninhibitor of growth family member 3
- Target synonymsEaf4; ING2; inhibitor of growth protein 3; MEAF4; p47ING3
- HostGoat
- IsotypeIgG
- Protein IDQ9NXR8
- Protein NameInhibitor of growth protein 3
- Scientific Descriptionp47 ING3 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203