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ProductsBack/iNOS antibody [4E5]
FACS analysis of MCF-7 cells using GTX60599 iNOS antibody [4E5]. Green : iNOS Red : negative control
FACS analysis of MCF-7 cells using GTX60599 iNOS antibody [4E5]. Green : iNOS Red : negative control
FACS analysis of MCF-7 cells using GTX60599 iNOS antibody [4E5]. Green : iNOS Red : negative control

iNOS antibody [4E5]

GTX60599
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNOS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    iNOS antibody [4E5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4843
  • Target name
    NOS2
  • Target description
    nitric oxide synthase 2
  • Target synonyms
    HEP-NOS, INOS, NOS, NOS2A, nitric oxide synthase, inducible, NOS, type II, hepatocyte NOS, inducible NO synthase, inducible NOS, nitric oxide synthase 2, inducible, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, macrophage, peptidyl-cysteine S-nitrosylase NOS2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35228
  • Protein Name
    Nitric oxide synthase, inducible
  • Scientific Description
    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Chromofungin, a chromogranin A-derived peptide, protects against sepsis-induced acute lung injury by inhibiting LBP/TLR4-dependent inflammatory signaling.
    Read this paper
  • Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
    Read this paper
  • Lupeol reduces M1 macrophage polarization to attenuate immunologic dissonance and fatty acid deposition in rats with diet-induced metabolic syndrome. Li J et al., 2021 Oct, Ann Transl Med
    Read this paper
  • Mesenchymal stem cells attenuate sepsis-induced liver injury via inhibiting M1 polarization of Kupffer cells. Liang X et al., 2019 Feb, Mol Cell Biochem
    Read this paper
  • Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model. Liu MC et al., 2017, PLoS One
    Read this paper
  • Effect of magnesium sulfate on renal ischemia-reperfusion injury in streptozotocin-induced diabetic rats. Akan M et al., 2016 Apr, Eur Rev Med Pharmacol Sci
    Read this paper

Datasheet

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Figure 1. Western blot analysis of NOS2 using anti-NOS2 antibody (A00368). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS2 antigen affinity purified polyclonal antibody (Catalog # A00368) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS2 at approximately 131 kDa. The expected band size for NOS2 is at 131 kDa.
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