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Western Blot analysis of SH-SY5Y cells using NOS2 Polyclonal Antibody
Western Blot analysis of SH-SY5Y cells using NOS2 Polyclonal Antibody
Western Blot analysis of SH-SY5Y cells using NOS2 Polyclonal Antibody

NOS2 Antibody

CSB-PA003464
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNOS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4843
  • Target name
    NOS2
  • Target description
    nitric oxide synthase 2
  • Target synonyms
    HEP-NOS, INOS, NOS, NOS2A, nitric oxide synthase, inducible, NOS, type II, hepatocyte NOS, inducible NO synthase, inducible NOS, nitric oxide synthase 2, inducible, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, macrophage, peptidyl-cysteine S-nitrosylase NOS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35228
  • Protein Name
    Nitric oxide synthase, inducible
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOS2 using anti-NOS2 antibody (A00368). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS2 antigen affinity purified polyclonal antibody (Catalog # A00368) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS2 at approximately 131 kDa. The expected band size for NOS2 is at 131 kDa.
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