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ProductsBack/iNOS antibody [N1N2-2], N-term

iNOS antibody [N1N2-2], N-term

GTX124210
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNOS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    iNOS antibody [N1N2-2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.83 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4843
  • Target name
    NOS2
  • Target description
    nitric oxide synthase 2
  • Target synonyms
    hepatocyte NOS; HEP-NOS; inducible NO synthase; inducible NOS; INOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS; NOS, type II; NOS2A; peptidyl-cysteine S-nitrosylase NOS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35228
  • Protein Name
    Nitric oxide synthase, inducible
  • Scientific Description
    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Macrophage polarity and wound age determination.
    Read more
  • The expressions of AMACR and iNOS in prostate adenocarcinomas. Ozgur T et al., 2013 Apr, Pak J Med Sci
    Read more

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Figure 1. Western blot analysis of NOS2 using anti-NOS2 antibody (A00368). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS2 antigen affinity purified polyclonal antibody (Catalog # A00368) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS2 at approximately 131 kDa. The expected band size for NOS2 is at 131 kDa.
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