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INPP5F Antibody (aa133-161)

LS-C162849
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetINPP5F
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    INPP5F Antibody (aa133-161)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID22876
  • Target name
    INPP5F
  • Target description
    inositol polyphosphate-5-phosphatase F
  • Target synonyms
    MSTP007, MSTPO47, SAC2, hSAC2, phosphatidylinositide phosphatase SAC2, Sac domain-containing inositol phosphatase 2, sac domain-containing phosphoinositide 4-phosphatase 2, sac domain-containing phosphoinositide 5-phosphatase 2
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunofluorescence staining of SH-SY5Y cells with CSB-PA896692NA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of INPP5F using anti-INPP5F antibody (A04954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5F antigen affinity purified polyclonal antibody (Catalog # A04954-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP5F at approximately 128 kDa. The expected band size for INPP5F is at 128 kDa.
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