More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/INPP5F Antibody
Immunofluorescence staining of SH-SY5Y cells with CSB-PA896692NA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of SH-SY5Y cells with CSB-PA896692NA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of SH-SY5Y cells with CSB-PA896692NA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

INPP5F Antibody

CSB-PA896692NA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetINPP5F
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    INPP5F Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22876
  • Target name
    INPP5F
  • Target description
    inositol polyphosphate-5-phosphatase F
  • Target synonyms
    MSTP007, MSTPO47, SAC2, hSAC2, phosphatidylinositide phosphatase SAC2, Sac domain-containing inositol phosphatase 2, sac domain-containing phosphoinositide 4-phosphatase 2, sac domain-containing phosphoinositide 5-phosphatase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2H2
  • Protein Name
    Phosphatidylinositide phosphatase SAC2
  • Scientific Description
    Inositol 4-phosphatase which mainly acts on phosphatidylinositol 4-phosphate. May be functionally linked to OCRL, which converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol, for a sequential dephosphorylation of phosphatidylinositol 4,5-bisphosphate at the 5 and 4 position of inositol, thus playing an important role in the endocytic recycling (PubMed:25869669). Regulator of TF:TFRC and integrins recycling pathway, is also involved in cell migration mechanisms (PubMed:25869669). Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation (PubMed:17322895). Negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus (PubMed:25476455). Functionally important modulator of cardiac myocyte size and of the cardiac response to stress (By similarity). May play a role as negative regulator of axon regeneration after central nervous system injuries (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
GeneTex
INPP5F antibodyGTX104273
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetINPP5F
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SAC2 (N-term) Antibody102-25547
ApplicationsWestern Blot
TargetINPP5F
  • SizePrice
Figure 1. Western blot analysis of INPP5F using anti-INPP5F antibody (A04954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5F antigen affinity purified polyclonal antibody (Catalog # A04954-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP5F at approximately 128 kDa. The expected band size for INPP5F is at 128 kDa.
Product group Antibodies
Boster Bio
Anti-INPP5F Antibody Picoband(r)A04954-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetINPP5F
  • SizePrice
Anti-INPP5F Antibody
Product group Antibodies
Antibodies.com
Anti-INPP5F AntibodyA34893
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Assay Genie
INPP5F AntibodyPACO62647
ApplicationsImmunoFluorescence, ELISA
TargetINPP5F
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
INPP5F Antibody (aa133-161)LS-C162849
ApplicationsWestern Blot
TargetINPP5F
  • SizePrice