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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with INPP5F antibody (GTX117067) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with INPP5F antibody (GTX117067) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with INPP5F antibody (GTX117067) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

INPP5F antibody

GTX117067
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetOCRL
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Overview

  • Supplier
    GeneTex
  • Product Name
    INPP5F antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4952
  • Target name
    OCRL
  • Target description
    OCRL inositol polyphosphate-5-phosphatase
  • Target synonyms
    DENT2, Dent-2, INPP5F, LOCR, NPHL2, OCRL-1, OCRL1, inositol polyphosphate 5-phosphatase OCRL, Lowe oculocerebrorenal syndrome protein, inositol polyphosphate 5-phosphatase OCRL-1, oculocerebrorenal syndrome of Lowe, phosphatidylinositol 3,4,5-triphosphate 5-phosphatase, phosphatidylinositol polyphosphate 5-phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01968
  • Protein Name
    Inositol polyphosphate 5-phosphatase OCRL
  • Scientific Description
    This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OCRL using anti-OCRL antibody (A02042-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCRL antigen affinity purified polyclonal antibody (Catalog # A02042-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCRL at approximately 104 kDa. The expected band size for OCRL is at 104 kDa.
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