Insulin antibody detects Insulin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas. Insulin stained by Insulin antibody (GTX27842) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Insulin antibody

GTX27842

ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityHuman, Mouse

TargetINS

Overview

Supplier
GeneTex
Product Name
Insulin antibody
Delivery Days Customer
9
Application Supplier Note
For IHC/ICC: Use at a dilution of 1:50-1:100. Prolonged fixation in buffered formalin can destroy the epitope. Optimal dilutions/concentrations should be determined by the end user.
Applications
ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Polyclonal
Concentration
0.2 mg/ml
Conjugate
Unconjugated
Gene ID3630
Target name
INS
Target description
insulin
Target synonyms
IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4, insulin, preproinsulin, proinsulin
Host
Guinea Pig
Isotype
IgG
Protein IDP01308
Protein Name
Insulin
Scientific Description
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Reactivity
Human, Mouse
Storage Instruction
2°C to 8°C
UNSPSC
12352203

References

Katz LS, Brill G, Wang P, et al. Transcriptional activation of the Myc gene by glucose in β-cells requires a ChREBP-dependent 3-D chromatin interaction between the Myc and Pvt1 genes. Mol Metab. 2024,79:101848. doi: 10.1016/j.molmet.2023.101848
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Daian LM, Tanko G, Vacaru AM, et al. Modulation of Unfolded Protein Response Restores Survival and Function of β-Cells Exposed to the Endocrine Disruptor Bisphenol A. Int J Mol Sci. 2023,24(3). doi: 10.3390/ijms24032023
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Morales-Reyes I, Atwater I, Esparza-Aguilar M, et al. Impact of biotin supplemented diet on mouse pancreatic islet β-cell mass expansion and glucose induced electrical activity. Islets. 2022,14(1):149-163. doi: 10.1080/19382014.2022.2091886
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Lee SJ, Kim HJ, Byun NR, et al. Donor-Specific Regulatory T Cell-Mediated Immune Tolerance in an Intrahepatic Murine Allogeneic Islet Transplantation Model with Short-Term Anti-CD154 mAb Single Treatment. Cell Transplant. 2020,29:963689720913876. doi: 10.1177/0963689720913876
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Chien HJ, Chiang TC, Peng SJ, et al. Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation. Am J Physiol Gastrointest Liver Physiol. 2019,317(5):G694-G706. doi: 10.1152/ajpgi.00116.2019
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Nteeba J, Kubota K, Wang W, et al. Pancreatic prolactin receptor signaling regulates maternal glucose homeostasis. J Endocrinol. 2019,241(1):71-83. doi: 10.1530/JOE-18-0518
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Tang SC, Shen CN, Lin PY, et al. Pancreatic neuro-insular network in young mice revealed by 3D panoramic histology. Diabetologia. 2018,61(1):158-167. doi: 10.1007/s00125-017-4408-y
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Tang SC, Baeyens L, Shen CN, et al. Human pancreatic neuro-insular network in health and fatty infiltration. Diabetologia. 2018,61(1):168-181. doi: 10.1007/s00125-017-4409-x
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Yamashita-Sugahara Y, Matsumoto M, Ohtaka M, et al. An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors. Sci Rep. 2016,6:35908. doi: 10.1038/srep35908
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Lin PY, Peng SJ, Shen CN, et al. PanIN-associated pericyte, glial, and islet remodeling in mice revealed by 3D pancreatic duct lesion histology. Am J Physiol Gastrointest Liver Physiol. 2016,311(3):G412-22. doi: 10.1152/ajpgi.00071.2016
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Insulin antibody

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