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ProductsBack/Integrin beta 1 / CD29 antibody [3B6]
IHC-P analysis of cervical cancer tissue using GTX60563 Integrin beta 1 / CD29 antibody [3B6].
IHC-P analysis of cervical cancer tissue using GTX60563 Integrin beta 1 / CD29 antibody [3B6].
IHC-P analysis of cervical cancer tissue using GTX60563 Integrin beta 1 / CD29 antibody [3B6].

Integrin beta 1 / CD29 antibody [3B6]

GTX60563
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey
TargetITGB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Integrin beta 1 / CD29 antibody [3B6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3B6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3688
  • Target name
    ITGB1
  • Target description
    integrin subunit beta 1
  • Target synonyms
    CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, integrin beta-1, glycoprotein IIa, integrin VLA-4 beta subunit, integrin beta 1, integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12), very late activation protein, beta polypeptide
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05556
  • Protein Name
    Integrin beta-1
  • Scientific Description
    Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Integrin Beta 1/ITGB1 using anti-Integrin Beta 1/ITGB1 antibody (A00772-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Beta 1/ITGB1 antigen affinity purified polyclonal antibody (Catalog # A00772-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Beta 1/ITGB1 at approximately 88,115-140 kDa. The expected band size for Integrin Beta 1/ITGB1 is at 88 kDa.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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WB analysis of various samples using GTX57718 Integrin beta 1 / CD29 antibody. Lane 1 : A549 whole cell lysate Lane 2 : PC-3 whole cell lysate Lane 3 : HT1080 whole cell lysate Loading : 40 μg Dilution : 1:1000
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