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ProductsBack/Anti-CD29 [K20]

Anti-CD29 [K20]

Ab00221-2.0
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetITGB1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD29 [K20]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antiobdy binds to CD29.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    IHC-F; FC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    K20
  • Gene ID3688
  • Target name
    ITGB1
  • Target description
    integrin subunit beta 1
  • Target synonyms
    CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, integrin beta-1, glycoprotein IIa, integrin VLA-4 beta subunit, integrin beta 1, integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12), very late activation protein, beta polypeptide
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP05556
  • Protein Name
    Integrin beta-1
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Human T-cell lymphoma cells injected into Biozzi high-responder mice.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Beta 1/ITGB1 using anti-Integrin Beta 1/ITGB1 antibody (A00772-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Beta 1/ITGB1 antigen affinity purified polyclonal antibody (Catalog # A00772-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Beta 1/ITGB1 at approximately 88,115-140 kDa. The expected band size for Integrin Beta 1/ITGB1 is at 88 kDa.
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