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ProductsBack/Integrin beta 3 (ITGB3/CD61) Rabbit mAb

Integrin beta 3 (ITGB3/CD61) Rabbit mAb

A19073
ABclonal
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetITGB3
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Overview

  • Supplier
    ABclonal
  • Product Name
    Integrin beta 3 (ITGB3/CD61) Rabbit mAb
  • Delivery Days Customer
    12
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID3690
  • Target name
    ITGB3
  • Target description
    integrin subunit beta 3
  • Target synonyms
    antigen CD61; BDPLT16; BDPLT2; BDPLT24; CD61; GP3A; GPIIIa; GT; GT2; integrin beta 3; integrin beta chain, beta 3; integrin beta-3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); platelet membrane glycoprotein IIIa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05106
  • Protein Name
    Integrin beta-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin beta 3/ITGB3 using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 3/ITGB3 antigen affinity purified polyclonal antibody (Catalog # A00587-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 3/ITGB3 at approximately 100 kDa. The expected band size for Integrin beta 3/ITGB3 is at 87 kDa.
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