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ProductsBack/IRAK4 Mouse Monoclonal Antibody [Clone ID: 6F8]
IHC staining of human FFPE tonsil section (Vermi William courtesy)
IHC staining of human FFPE tonsil section (Vermi William courtesy)
IHC staining of human FFPE tonsil section (Vermi William courtesy)

IRAK4 Mouse Monoclonal Antibody [Clone ID: 6F8]

DDX0340A488-100
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetIRAK4
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    IRAK4 Mouse Monoclonal Antibody [Clone ID: 6F8]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6f8
  • Gene ID51135
  • Target name
    IRAK4
  • Target description
    interleukin 1 receptor associated kinase 4
  • Target synonyms
    IMD67; interleukin-1 receptor-associated kinase 4; IPD1; IRAK-4; NY-REN-64; REN64; renal carcinoma antigen NY-REN-64
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    IRAK4 mouse monoclonal antibody, clone 6F8
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRAK4 using anti-IRAK4 antibody (A01247-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRAK4 antigen affinity purified polyclonal antibody (Catalog # A01247-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRAK4 at approximately 52 kDa. The expected band size for IRAK4 is at 52 kDa.
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Lane 1: Mouse Muscle lysates; Lane 2: Mouse Stomach lysates; Lane 3: Rat Muscle lysates probed with IRAK4 Polyclonal Antibody, Unconjugated (bs-2440R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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