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Western Blot Positive WB detected in: Mouse liver tissue All lanes: IRF9 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: IRF9 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: IRF9 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa

IRF9 Antibody

CSB-PA011824LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetIRF9
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Overview

  • Supplier
    Cusabio
  • Product Name
    IRF9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10379
  • Target name
    IRF9
  • Target description
    interferon regulatory factor 9
  • Target synonyms
    IRF-9, ISGF3, ISGF3G, p48, interferon regulatory factor 9, IFN-alpha-responsive transcription factor subunit, ISGF-3 gamma, ISGF3 p48 subunit, interferon-stimulated gene factor 3 gamma, interferon-stimulated transcription factor 3, gamma (48kD), interferon-stimulated transcription factor 3, gamma 48kDa, transcriptional regulator ISGF3 subunit gamma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00978
  • Protein Name
    Interferon regulatory factor 9
  • Scientific Description
    Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of IRF9 using anti-IRF9 antibody (A04485). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: JURKAT cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF9 antigen affinity purified polyclonal antibody (Catalog # A04485) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF9 at approximately 44KD. The expected band size for IRF9 is at 44KD.
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