Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE GTX113213 diluted at 1:10000
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRS1 antibody [C2C3], C-term (GTX113213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113213/GTX113213_40394_20170810_WB_w_23060501_929.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRS1 antibody [C2C3], C-term (GTX113213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![IRS1 antibody [C2C3], C-term detects IRS1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung carcinoma. IRS1 antibody [C2C3], C-term (GTX113213) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX113213/GTX113213_40394_IHC_w_23060501_262.webp "IRS1 antibody [C2C3], C-term detects IRS1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung carcinoma. IRS1 antibody [C2C3], C-term (GTX113213) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE GTX113213 diluted at 1:10000
IRS1 antibody [C2C3], C-term
GTX113213
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIRS1
Overview
- SupplierGeneTex
- Product NameIRS1 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3667
- Target nameIRS1
- Target descriptioninsulin receptor substrate 1
- Target synonymsHIRS-1, insulin receptor substrate 1
- HostRabbit
- IsotypeIgG
- Protein IDP35568
- Protein NameInsulin receptor substrate 1
- Scientific DescriptionThis gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chang TC, Chiou WC, Lai WH, et al. Ugonin J improves metabolic disorder and ameliorates nonalcoholic fatty liver disease by regulating the AMPK/AKT signaling pathway. Pharmacol Res. 2021,163:105298. doi: 10.1016/j.phrs.2020.105298Read this paper
Datasheet
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