WB (peptide competition) analysis of CHO-T cells transiently transfected with wild-type human IRS-1 and treated with 100 ng/mL TPA for 30 minutes using GTX24865 IRS1 (phospho Ser312) antibody prior incubated with no peptide (Lane 1~3) , the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), a generic phosphoserine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of CHO-T cells transiently transfected with wild-type human IRS-1 and treated with 100 ng/mL TPA for 30 minutes using GTX24865 IRS1 (phospho Ser312) antibody prior incubated with no peptide (Lane 1~3) , the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), a generic phosphoserine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.
IRS1 (phospho Ser312) antibody
GTX24865
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetIRS1
Overview
- SupplierGeneTex
- Product NameIRS1 (phospho Ser312) antibody - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID3667
- Target nameIRS1
- Target descriptioninsulin receptor substrate 1
- Target synonymsHIRS-1; insulin receptor substrate 1
- HostRabbit
- IsotypeIgG
- Protein IDP35568
- Protein NameInsulin receptor substrate 1
- Scientific DescriptionThis gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hydrogen Sulphide Treatment Increases Insulin Sensitivity and Improves Oxidant Metabolism through the CaMKKbeta-AMPK Pathway in PA-Induced IR C2C12 Cells. Chen X et al., 2017 Oct 16, Sci RepRead more