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IRS1 (phospho Ser312) antibody

GTX79036
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetIRS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    IRS1 (phospho Ser312) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3667
  • Target name
    IRS1
  • Target description
    insulin receptor substrate 1
  • Target synonyms
    HIRS-1; insulin receptor substrate 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35568
  • Protein Name
    Insulin receptor substrate 1
  • Scientific Description
    This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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WB (peptide competition) analysis of CHO-T cells transiently transfected with wild-type human IRS-1 and treated with 100 ng/mL TPA for 30 minutes using GTX24865 IRS1 (phospho Ser312) antibody prior incubated with no peptide (Lane 1~3) , the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 4), a generic phosphoserine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6) . The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.
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