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Lane 1: HeLa; Lane 2: MCF-7 cell lysate at 20 µg per lane, probed with bsm-51373M ISG15 (1031CT2.8.3) Monoclonal Antibody at 1:8000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: HeLa; Lane 2: MCF-7 cell lysate at 20 µg per lane, probed with bsm-51373M ISG15 (1031CT2.8.3) Monoclonal Antibody at 1:8000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: HeLa; Lane 2: MCF-7 cell lysate at 20 µg per lane, probed with bsm-51373M ISG15 (1031CT2.8.3) Monoclonal Antibody at 1:8000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

ISG15 (1C3) Monoclonal Antibody

BSM-51373M
Bioss
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetISG15
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Overview

  • Supplier
    Bioss
  • Product Name
    ISG15 (1C3) Monoclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1C3
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID9636
  • Target name
    ISG15
  • Target description
    ISG15 ubiquitin like modifier
  • Target synonyms
    G1P2, IFI15, IMD38, IP17, UCRP, hUCRP, ubiquitin-like protein ISG15, interferon, alpha-inducible protein (clone IFI-15K), interferon-induced 17-kDa/15-kDa protein, interferon-stimulated protein, 15 kDa, ubiquitin cross-reactive protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05161
  • Protein Name
    Ubiquitin-like protein ISG15
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ISG15 using anti-ISG15 antibody (PB9950). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates, Lane 2: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISG15 antigen affinity purified polyclonal antibody (Catalog # PB9950) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ISG15 at approximately 15 kDa. The expected band size for ISG15 is at 18 kDa.
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